t mk2  (Cell Signaling Technology Inc)

Journal: Current Issues in Molecular Biology

Article Title: Mitigation of 3.5 GHz Electromagnetic Field-Induced BV2 Microglial Cytotoxicity by Polydeoxyribonucleotide

doi: 10.3390/cimb47060386

Figure Lengend Snippet: Effect of SP600125, SB203580, or PD98059 on 3.5 GHz EMF-induced growth suppression of BV2 cells and phosphorylation of JNK-1/2, p38 MAPK (MK2), or ERK-1/2 in BV2 cells. ( A ) BV2 cells were exposed to 3.5 GHz EMFs without or with SP600125 (25 μM), a JNK-1/2 inhibitor, SB203580 (25 μM), a p38 MAPK inhibitor, or PD98059 (50 μM), an ERK-1/2 inhibitor for 2 h. The number of surviving cells was analyzed using cell count analysis. Data represent the mean ± SE of three independent experiments. * p < 0.01 compared with the values of the control (no 3.5 GHz EMF exposure). # p < 0.01 compared with the values of 3.5 GHz EMF exposure (no drug), as determined by one-way ANOVA followed by Sidak’s post hoc test. ( B ) A representative image of morphological changes in the conditioned cells in ( A ). ( C ) BV2 cells were exposed to 3.5 GHz EMFs without or with SP600125 (25 μM), SB203580 (25 μM), or PD98059 (50 μM) for 2 h. Whole-cell lysates from the conditioned cells were prepared and analyzed using Western blotting with antibodies.

Article Snippet: The primary antibodies, including eukaryotic initiation factor-2α (eIF-2α) (cat. no. 9722), phosphorylated (p)-extracellular signal-regulated protein kinase-1/2 (p-ERK-1/2) (T202/Y204) (cat. no. 9101), ERK-1/2 (cat. no. 9102), p-JNK-1/2 (T183/Y185) (cat. no. 9251), JNK-1/2 (cat. no. 9252), and p-p38 MAPK (T180/Y182) (cat. no. 9211) p38 MAPK (cat. no. 9212), p-MK2 (T334) (cat. no. 3007), and T-MK2 (cat. no. 3042), were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Phospho-proteomics, Cell Counting, Control, Western Blot

Journal: Frontiers in Immunology

Article Title: Intranasal MMI-0100 Attenuates Aβ 1−42 - and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway

doi: 10.3389/fimmu.2019.02707

Figure Lengend Snippet: Information on antibodies used in this experiment.

Article Snippet: Anti-p-MK2 Rabbit mAb Anti-t-MK2 Rabbit mAb Anti-p-p38 Rabbit mAb , 1:1,000 1:1,000 1:1,000 , 3700S D16188-0025 4511S , CST, USA BBI Life Science CST, USA.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Intranasal MMI-0100 Attenuates Aβ 1−42 - and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway

doi: 10.3389/fimmu.2019.02707

Figure Lengend Snippet: Effect of MMI-0100 on Aβ 1−42 - or LPS-induced activation of MAPK in the hippocampus in mice. The upper panel is a representative immunoblot of pERK, tERK, pJNK, tJNK, p38, and p-p38 detected by Western blotting; the lower panel is quantification of MAPK signaling pathway-related protein expressed as the ratio of pERK/tERK, pJNK/tJNK, and p-p38/p38. Values were expressed as mean ± SEM, n = 5 in each group. ** p < 0.01 and *** p < 0.001 compared with vehicle group.

Article Snippet: Anti-p-MK2 Rabbit mAb Anti-t-MK2 Rabbit mAb Anti-p-p38 Rabbit mAb , 1:1,000 1:1,000 1:1,000 , 3700S D16188-0025 4511S , CST, USA BBI Life Science CST, USA.

Techniques: Activation Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: Intranasal MMI-0100 Attenuates Aβ 1−42 - and LPS-Induced Neuroinflammation and Memory Impairments via the MK2 Signaling Pathway

doi: 10.3389/fimmu.2019.02707

Figure Lengend Snippet: Effects of MMI-0100 treatment on LPS-induced inflammatory response and the expression of pMK2 in BV-2 cells. (A) MMI-0100 reduces LPS (1 μg/ml)-induced release of pro-inflammatory mediators such as IL-6, IL-1β, TNF-α, COX-2, and iNOS level in BV-2 cells. (B) The upper panel is a representative immunoblot of pMK2, tMK2, and GAPDH detected by Western blotting; the lower panel is quantification of MK2 expressed as the ratio of pMK2/tMK2. ** p < 0.01 compared with vehicle group vs LPS group. # p < 0.05 and ## p < 0.01 compared with the LPS group vs. LPS + MMI-0100 (10 −5 , 10 −6 , 10 −7 , and 10 −8 M) at different doses. Values were expressed as mean ± SEM.

Article Snippet: Anti-p-MK2 Rabbit mAb Anti-t-MK2 Rabbit mAb Anti-p-p38 Rabbit mAb , 1:1,000 1:1,000 1:1,000 , 3700S D16188-0025 4511S , CST, USA BBI Life Science CST, USA.

Techniques: Expressing, Western Blot